Journal of Agricultural Research (YAU-JAR)

Journal of Agricultural Research (2020), Volume. 7, Issue (2)

Morphological and Biochemical Characterization of Indigenous Rhizobium Isolates from Root Nodules of Green Gram (Vigna radiata L.)

Kyi Kyi Thein, Myat Lin, Ei Ei Aung, Aye Aye Than, Tin Aye Aye Naing

Abstract

The present study was conducted at Department of Plant Pathology, Yezin Agricultural University from August to September 2018. A total of 14 indigenous rhizobium isolates with higher symbiotic effectiveness were morphologically and biochemically characterized from root nodules of green gram variety 'Yezin-11´. All the isolates grew well on Yeast Extract Mannitol Agar (YMA) medium showing typical characters of rhizobia. Out of 14 isolates, 7 were selected as fast-growing Rhizobium spp. and the rest as slow-growing Bradyrhizobium spp. based on Bromothymol Blue (BTB) test. All the 14 rhizobium isolates were found gram-negative and rod-shaped. All the green gram rhizobium isolates examined were able to grow in 2% NaCl and also utilize glucose as a sole source of carbon. Starch hydrolysis test was negative except for M-1, P-9, M-12 and P-8. Positive results were found with catalase and urease tests for all isolates. None of the isolates except P-15 showed positive reaction to Methyl Red (MR) test.